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Recombinant Rabbit Monoclonal
Anti-Ubiquityl-Histone H2A (Lys119) Rabbit mAb
Catalog Number: PTM-7449
$ 330

Clone Number: JMMR-N1208

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB ICC/IF IP ChIP

Reactivity: Human, Mouse

Synonyms: H2AK119ub

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype
Conjugate Unconjugated
Synonyms H2AK119ub
UniProt ID

P04908

Immunogen Ubiquitinated human histone H2A (Lys119) peptide
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
Properties
Purity
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Cellular location

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: NIH-3T3, 3: C2C12
Protein loading amount: 20 μg
Exposure time: 3 s
Predicted MW: 23 kDa
Observed MW: 23 kDa

ICC/IF

Cell line: HeLa
Fixation: 4% PFA
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Primary Ab incubation condition: 4℃ overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (red)
Comment: P

IP

P of HeLa cells extracts:
IP Ab incubation condition: PTM-7449, 4°C overnight, 1:100 dilution
WB primary Ab incubation condition: PTM-7449, room temperature 2h , 1:1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
1: 5%% Input
2: IP with Rabbit monoclonal IgG Isotype
3: IP with PTM-7449
Observed MW: 23 kDa
Exposure time: 30 s

Research Use

For research use only, not for use in diagnostic procedures.

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