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Recombinant Rabbit Monoclonal
Anti-GATA1 Rabbit mAb
Catalog Number: PTM-6994
$ 280

Clone Number: JMMR-3148

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IHC-P

Reactivity: Human

Synonyms: ERYF1, GF1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms ERYF1, GF1
UniProt ID

P15976

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 Human
IHC-P 1:500 - 1:2000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

GATA1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development. Mutations in the corresponding GATA1 gene are linked to many human diseases, including acute megakaryoblastic leukemia in Down Syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate)
Lysate: 1: HeLa (Negative control), 2: K562, 3: TF-1
Protein loading quantity: 20 μg
Exposure time: 10 seconds
Predicted band size: 43 kDa
Observed band size: 53 kDa

IHC-P

Tissue: Human colon
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:2000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6994

Tissue: Human pancreas (Negative tissue)
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:2000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: No staining on Human pancreas

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 280
    Recombinant Rabbit Monoclonal
    Anti-GATA1 Rabbit mAb
    Catalog #
    PTM-7444
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P