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Anti-D-Lactyllysine Rabbit pAb
Catalog Number: PTM-1429
$ 435

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB

Reactivity: All

Synonyms: Kla, D-la

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kla, D-la
UniProt ID

/

Immunogen D-lactylated lysine peptides
MW (kDa) Multiple
Specificity Anti-D-Lactyllysine Rabbit pAb detects proteins with D-lactylated lysine residues, with no cross-reactivity to its isomers L-lactyllysine or N-ε-(carboxyethyl)-lysine (Kce). This pan antibody recognizes D-lactylated lysine regardless of the surrounding amino acid sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1500 All
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

D-lactyllysine (KD-la) refers to the modification of lysine residues in proteins by the addition of D-lactate.dues in proteins. This modification was first reported by Zhang et al. in 2024, with the findings published in Nature Chemical Biology. KD-la forms through a non-enzymatic reaction between proteins and S-D-lactoylglutathione (LGSH), which is a byproduct of the glyoxalase pathway. This pathway involves two key enzymes: glyoxalase 1 (GLO1) and glyoxalase 2 (GLO2). GLO1 conjugates methylglyoxal (MGO), a glycolysis byproduct, with glutathione to form LGSH, which is hydrolyzed by GLO2 to produce D-lactate while regenerating cellular glutathione. In humans, L-lactate is a common metabolic product that plays a key role in energy metabolism, especially during muscle activity. In contrast, D-lactate is not a typical product of human metabolism but is mainly produced by microorganisms such as certain bacteria and yeasts during fermentation. The potential harm of D-lactate mainly lies in its accumulation under specific conditions, such as intestinal microbiota dysbiosis, short bowel syndrome, or certain disease states. Excessive production of D-lactate can lead to D-lactic acidosis, characterized by symptoms of acidosis (low blood bicarbonate levels, decreased blood pH, and hyperuricemia), and in severe cases, neurological symptoms like seizures, ataxia, and altered consciousness.

Cellular location

/

Images
Dot Blot

Peptide amount: 10 ng, 20 ng, 50 ng, 100 ng, 500 ng
Blocking buffer: 3% BSA/TBST
Primary Ab dilution: 1:1500
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 15 seconds
The list of peptides used in the experiment is provided below.
Kce: Carboxyethylated peptide library. KD-la: D-lactylated peptide library.
KL-la: L-lactylated peptide library. Kac: Acetylated peptide library.
K: Unmodified peptide library.

Peptide amount: 10 ng, 20 ng, 50 ng, 100 ng
Blocking buffer: 3% BSA/TBST
Primary Ab dilution: 1:1500
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 15 seconds
The peptides used in the experiment are synthetic, sequence-specific histone peptides with the following modifications:
KL-la: L-lactylated peptide library. KD-la: D-lactylated peptide library.
Kce: Carboxyethylated peptide library.

WB

Samples: BSA, Acetylated BSA, L-lactylated BSA, D-lactylated BSA, Carboxyethylated BSA
Blocking buffer: 3% BSA/TBST
Primary Ab dilution: 1:1500
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 15 seconds

Lysates: MCF-7 cells treated with 0.1, 1, 5, and 25 mM glucose; MCF-7 cells treated with 0, 0.1, 0.5, and 2.5 µM enolase inhibitor POMHEX; MCF-7 cells treated with 0, 0.5, 2.5, and 10 µM lactate dehydrogenase inhibitor (R)-GNE-140
Blocking buffer: 3% BSA/TBST
Primary Ab dilution: 1:1500
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted MW: Multiple
Observed MW: Multiple

Lysates: HEK-293T, GLO1 knockout HEK-293T, GLO2 knockout HEK-293T, and GLO1/2 knockout HEK-293T cells treated with 0.1, 1, 5, 25 mM glucose
Blocking buffer: 3% BSA/TBST
Primary Ab dilution: 1:1500
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: Multiple
Observed band size: Multiple

Research Use

For research use only, not for use in diagnostic procedures.

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