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Recombinant Rabbit Monoclonal
Anti-COUP-TFII Rabbit mAb
Catalog Number: PTM-7113
$ 290

Clone Number: JRMR-11053-2

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IHC-P ChIP

Reactivity: Human, Mouse, Rat

Synonyms: COUP transcription factor 2, COUP-TF2, Apolipoprotein A-I regulatory protein 1 (ARP-1), COUP transcription factor II (COUP-TF II), Nuclear receptor subfamily 2 group F member 2, NR2F2, ARP1, TFCOUP2

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms COUP transcription factor 2, COUP-TF2, Apolipoprotein A-I regulatory protein 1 (ARP-1), COUP transcription factor II (COUP-TF II), Nuclear receptor subfamily 2 group F member 2, NR2F2, ARP1, TFCOUP2
UniProt ID

P24468

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Cellular location

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: MCF-7, 2: K562, 3: PC-12
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 46 kDa
Observed MW: 46 kDa

IHC-P

Tissue: Human testis
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Comment: Color brown is the positive signal for PTM-7113

Tissue: Human breast carcinoma
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Comment: Color brown is the positive signal for PTM-7113

Tissue: Mouse testis
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Comment: Color brown is the positive signal for PTM-7113

Tissue: Rat testis
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Comment: Color brown is the positive signal for PTM-7113

Research Use

For research use only, not for use in diagnostic procedures.