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Anti-Citrulline-NPM1 (Arg196) Rabbit pAb
Catalog Number: PTM-7073
$ 435

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB

Reactivity: Human

Synonyms: Numatrin, NMP, NMP1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Numatrin, NMP, NMP1
UniProt ID

P06748

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
IHC-P 1:1000 - 1:2000 Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Nucleophosmin (NPM1) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. NPM1 is involved in ribosomal assembly and rRNA transport. It is highly phosphorylated by Cdc2 kinase during mitosis.

Cellular location

Nucleus, nucleoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: (-): HEK-293T transfected with a control vector containing GFP tag, treatedwith10 mM calcium chloride and 10 µM Ionomycin for 2 hours; (+): HEK-293T transfectedwithGFP-tagged PADI2 (WT) expression vector, treated with 10 mM calciumchlorideand10µM Ionomycin for 2 hours
Protein loading amount: 20 μg
Exposure time: 10 seconds
Predicted band size: 33 kDa
Observed band size: 38 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: (-): HL-60 + DMSO(1.25%, 3-6d), (+): HL-60 + DMSO(1.25%, 3-6d) +A23187(4uM, 15min)
Protein loading amount: 20 μg
Exposure time: 30 seconds
Predicted band size: 33 kDa
Observed band size: 38 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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