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Recombinant Rabbit Monoclonal
Anti-Butyryl-Histone H3 (Lys9) Rabbit mAb
Catalog Number: PTM-312RM
$ 420

Clone Number: JMMR-P2630

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IP ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H3K9bu

Product Size
100μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K9bu
UniProt ID

P68431

Immunogen Butyrylated human histone H3 (Lys9) peptide
MW (kDa) 15
Specificity Anti-Butyryl-Histone H3 (Lys9) Rabbit mAb detects histone H3 when it is butyrylated at Lys9. 
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000-1:5000 All
IP 1:100-1:500 Human
ChIP 6 μg per 5x106 cells Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): HeLa, (+): HeLa+SBA(5mM, 24h)
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): NIH-3T3, (+): NIH-3T3+SBA(30mM, 4h)
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): C6, (+): C6+SBA(5mM, 24h)
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
IP

IP of HeLa+SBA(5mM, 24h) cells extracts:
IP Ab incubation condition: PTM-312RM, 4°C overnight, 1:100 dilution
WB primary Ab incubation condition: PTM-312RM, room temperature 2h , 1:1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
1: 10%% Input
2: IP with PTM-312RM
3: IP with Rabbit monoclonal IgG Isotype
Observed MW: 15 kDa
Exposure time: 10 s

IP of HeLa cells extracts:
IP Ab incubation condition: PTM-312RM, 4°C overnight, 1:100 dilution
WB primary Ab incubation condition: PTM-312RM, room temperature 2h , 1:1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
1: 10% Input
2: IP with PTM-312RM
3: IP with Rabbit monoclonal IgG Isotype
Observed MW: 15 kDa
Exposure time: 10 s
ChIP

Cell type: HeLa+SBA(5μM,16h)+TSA(4μM, 16h)
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μl of Protein A MagBeads
Comment: The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH-CDS, RPL30, TuBBP10, LDHA promoter, LDHA-CDS, Foxo3a-promoter, Foxo3a-D and RAB20. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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