PTM BIO

中 / EN

Back

QUICK ORDER

Home
Services

Bioinformatics

Standard Bioinformatic Analysis

Customized Data Analysis

PTM Cloud

Proteomics

General Proteomic Analysis

Profiling of Post-translational Modifications

Featured Proteomics Sevice

Instrument and Equipment

Drug R&D Services

Drug Discovery

Preclinical Research

Protein Sequencing
Products

Research Antibody Products

FILTERED BY PRODUCT CATEGORY

Primary Antibodies

Pan PTM Antibodies

Histone and Histone Modification Antibodies

Organelle Marker Antibodies

Loading Control Antibodies

Epitope Tag Antibodies

Secondary Antibodies

FILTERED BY RESEARCH

Cancer

Cardiovascular Research

Cell Biology

Developmental Biology

Epigenetics

Immunology

Metabolism

Microbiology

Neuroscience

Post-Translational Modification

Signal Transduction

Stem Cells

IVD Products

Cardiac Markers

Tumor Markers

Diabetes and Metabolism

Thyroid Diseases

Hormone

Inflammation

Kidney Diseases

Bone Metabolism

Liver Diseases

Hypertension, Rheumatoid, Anemia

Fertility and Pregnancy, Pre-eclamps

Alzheimer's Disease

Infectious Diseases

Drugs of Abuse

Serology

Veterinary

Blood Clotting

Pathology

Antigen

Others

Custom Antibody Development
Support

Technical Support FAQ
About Us

Our Company Scientific Advisory Board News & Events Contact Us

Technical Support

| Contact Us

+1 312-802-6843

Technical Support

+1 312-802-6843

中 / EN

QUICK ORDER

Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development

Recover password

success

Recombinant Rabbit Monoclonal

Anti-Acetyl-Histone H4 (Lys77) Rabbit mAb

Catalog Number: PTM-127RM

$ 365

Clone Number: PA-086-13

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P IP ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H4K77ac

Product Size

100 μl

Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Product Usage Information
Properties
Target Information
Images
Recommended Products
References

BUY NOW

General Information

Isotype	IgG
Conjugate	Unconjugated
Synonyms	H4K77ac
UniProt ID	P62805
Immunogen	Acetylated human histone H4 (Lys77) peptide
MW (kDa)	11
Specificity	Anti-Acetyl-Histone H4 (Lys77) Rabbit mAb detects histone H4 only when it is acetylated at Lys77.

Product Usage Information

Applications	Dilution	Recommended Species
WB	1:500 - 1:2000	Human, Rat
IHC-P	1:200 - 1:1000	Human, Mouse, Rat
IP	1:50 - 1:100	Human
ChIP	6 μg per 5x10⁶ cells	Human

Properties

Purity	Protein A purified
Constituents	PBS, Glycerol, BSA
Storage	Store at -20°C. Avoid freeze/thaw cycles.
Stability	Stable for 12 months from date of receipt/reconstitution.

Target Information

Background

Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5, 12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, and chromatin dynamics. Recent research has identified that H2BK120ac, H3.3K18ac, and H4K77ac are significantly associated with the survival of hepatocellular carcinoma (HCC) patients. Furthermore, H4K77ac has been linked to HCC recurrence. These findings suggest that H2BK120ac, H3.3K18ac, and H4K77ac may be potential prognostic factors for HCC.

Cellular location

Nucleus

Images

Dot Blot
	Peptide amount: 1 ng, 4 ng, 16 ng, 64 ng Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided below. Lane 1: H4K77 acetyl. Dot 2: H4K77 2-hydroxyisobutyryl. Dot 3: H4K77 propionyl. Dot 4: H4K77 butyryl . Dot 5: H4K79 acetyl. Dot 6: H4K77 unmodified.

Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng, 64 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H4K77 acetyl. Dot 2: H4K77 2-hydroxyisobutyryl.
Dot 3: H4K77 propionyl. Dot 4: H4K77 butyryl .
Dot 5: H4K79 acetyl. Dot 6: H4K77 unmodified.

WB
	Lysates: (-) HeLa cells; (+) HeLa cells treated with 30 mM sodium butyrate for 4 hours Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa
	Lysates: 1. MCF-7 cells; 2. BRL cells; 3. recombinant histone H4 Protein loading amount: 20 μg for MCF-7 and BRL cell lysates; 20 ng for recombinant histone H4 Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa

Lysates: (-) HeLa cells; (+) HeLa cells treated with 30 mM sodium butyrate for 4 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa

Lysates: 1. MCF-7 cells; 2. BRL cells; 3. recombinant histone H4
Protein loading amount: 20 μg for MCF-7 and BRL cell lysates; 20 ng for recombinant histone H4
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa

IHC-P
	Tissue: Human kidney Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with PTM-127RM.
	Tissue: Human liver Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with PTM-127RM.
	Tissue: Human testis Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with PTM-127RM.
	Tissue: Mouse colon Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with PTM-127RM.
	Tissue: Rat liver Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with PTM-127RM.

IP
	IP of whole cell lysates from HeLa cells treated with 30 mM sodium butyrate for 4 hours Lane 1: 5% Input Lane 2: IP with PTM-127RM Lane 3: IP with Rabbit mAb IgG Isotype Control IP Ab incubation: 1:100 dilution, 4°C overnight Blocking buffer: 5% NFDM/TBST Dilution buffer: 5% NFDM/TBST WB Primary Ab incubation: PTM-127RM, 1:500 dilution, 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG for IP (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa

IP of whole cell lysates from HeLa cells treated with 30 mM sodium butyrate for 4 hours
Lane 1: 5% Input
Lane 2: IP with PTM-127RM
Lane 3: IP with Rabbit mAb IgG Isotype Control
IP Ab incubation: 1:100 dilution, 4°C overnight
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
WB Primary Ab incubation: PTM-127RM, 1:500 dilution, 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG for IP (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa

ChIP
	Sample: HeLa cells treated with 5 mM sodium butyrate for 24 hours Cross-linking conditions: no cross-linking Amount of chromatin per IP: 5×10⁶ cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A MagBeads Description: Chromatin immunoprecipitations were performed with 6 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human RPL30, FOXO3a promoter, FOXO3a downstream, and TUBBP10 regions. The data are presented as enrichment of each sample relative to the total amount of input chromatin at each amplicon.

ChIP

Sample: HeLa cells treated with 5 mM sodium butyrate for 24 hours
Cross-linking conditions: no cross-linking
Amount of chromatin per IP: 5×10⁶ cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μL of Protein A MagBeads
Description: Chromatin immunoprecipitations were performed with 6 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human RPL30, FOXO3a promoter, FOXO3a downstream, and TUBBP10 regions. The data are presented as enrichment of each sample relative to the total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.