Applications：
WB
IHC-P
ICC/IF
FC
IAP
IP
ChIP
mIHC-P
Clone Number: /
Host: Rabbit Clonality: Polyclonal
Applications： WB ChIP
Reactivity: Human, Mouse, Rat
Synonyms: H2AK9ac
Shipping: Ambient temperature
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| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | H2AK9ac |
| UniProt ID | |
| Immunogen | Acetylated human histone H2A (Lys9) peptide |
| MW (kDa) | 14 |
| Specificity | Anti-Acetyl-Histone H2A (Lys9) Rabbit pAb detects histone H2A only when it is acetylated at Lys9. This antibody has been shown to selectively recognize acetylated H2A peptide at Lys9, but not the structurally similar 2-hydroxyisobutyrylated, β-hydroxybutyrylated, succinylated H2A peptides at Lys5 or Lys9. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:500 - 1:2000 | Human, Mouse, Rat |
| ChIP | 4 ug/ 5×106 cells | Human |
| Purity | Antibodies are purified by protein A-conjugated agarose followed by Acetylated H2A (Lys9) peptide affinity hromatography |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation
Cellular location
Nucleus
| Dot Blot | |
|---|---|
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Peptide amount: 1 ng, 4 ng, 16 ng |
| WB | |
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Lysate: (-) HeLa; (+) HeLa + sodium butyrate (30 mM, 4 hours) |
| ChIP | |
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Cell type: HeLa cells + sodium butyrate (5 mM, 24 hours) |
Research Use
For research use only, not for use in diagnostic procedures.
